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. 2023 Mar 15;18(11):2449–2458. doi: 10.4103/1673-5374.371370

Figure 6.

Figure 6

Sirt6 overexpression ameliorates neurobehavioral deficits in chronic sleep deprived mice.

(A) Left: Representative traces for the open field test. Right: Quantitative results of total distance moved and the amount of time spent in the center zone. Chronic sleep deprived mice stayed for less time in the center zone, and Sirt6 overexpression in the PrL reversed CSD-induced anxiety-like behaviors. (B) Left: Representative traces for novel object recognition. Right: Qualification of the recognition index and entries into a novel object in each group. Chronic sleep deprivation reduced the recognition index and number of entries into a novel object compared with the control mice. Forced Sirt6 expression diminished chronic sleep deprivation-induced abnormalities in memory processes. (C) Left: Representative paths for the Y-maze test. Right: Quantitative results of averaged speed and novel arm preference index. Chronic sleep deprivation induced spatial memory impairments, as evidenced by a decrease in the novel arm preference index compared with control mice. Sirt6 overexpression prevented the decline in the novel arm preference index. (D) Left: Representative tracing heatmaps. Right: Quantitative results of the social preference index in the social session and the social novelty preference index in the social novelty session. Chronic sleep deprived mice spent a similar amount of time investigating the cup containing Stranger I and the empty wire cup. However, Sirt6-overexpressing chronic sleep deprived mice behaved similarly to control mice in that they tended to explore the cup containing Stranger I in the sociality session. Data are presented as mean ± SEM, and were analyzed by two-way analysis of variance followed by Tukey’s post hoc test. Red dot: start recording point; blue dot: end recording point; yellow line: the recorded areas; purple line: the movement locus of animals. CSD: Chronic sleep deprivation; eGFP: enhanced green fluorescent protein; F: familiar arm; N: novel arm; PrL: prelimbic cortex; S1 and S2: Stranger SI and II; Sirt6: Sirtuins 6.