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. 2023 Mar 31;18(11):2526–2534. doi: 10.4103/1673-5374.371372

Figure 2.

Figure 2

Expression of caRheb markedly promotes axon regeneration in ONC mice.

(A, B) Rheb induced robust axon regeneration. Images of optic nerve sections from AAV-caRheb and AAV-EGFP groups showing regenerating axons labeled by anterograde transport of CTB on days 14, 21, and 42 after ONC. Red asterisk indicates the crush site. Scale bars: 100 µm. (C) Quantification of regenerating axons 14 days post-crush from mice intravitreally injected with AAV-EGFP or AAV-caRheb at different distances distal to the lesion site (mean ± SD, n = 6–9). *P < 0.05, vs. AAV-EGFP group (two-way analysis of variance with Bonferroni’s post hoc test). AAV: Adeno-associated virus; caRheb: constitutively active Ras homolog enriched in brain; CTB: cholera toxin B subunit; dpc: days post crush; EGFP: enhanced green fluorescent protein; ONC: optic nerve crush; RGC: retinal ganglion cell.