Figure 1.

NBP improves cell viability and inhibits apoptosis in H₂O₂-treated cells.

(A) PC12 cells were treated with 200 µM H₂O₂ for 4 hours. The cells were pretreated with 20 and 40 µM NBP for 24 hours. CCK8 assay was used to explore the effect of NBP on cell viability. (B) PC12 cells were treated with 200 µM H₂O₂ for 4 hours. The cells were pretreated with 40 µM NBP for 24 hours. EdU was used to examine the effects of NBP on cell proliferation (original magnification, 100×). (C) Caspase-3 activity assay in PC12 cells (original magnification, 100×). Data are presented as mean ± SD. The experiment was repeated three times. *P < 0.05, **P < 0.01, ****P < 0.0001 (one-way analysis of variance followed by Bonferroni correction). CCK8: Cell Counting Kit-8; CTL: control; EdU: 5-ethynyl-2′-deoxyuridine; NBP: Dl-3-n-butylphthalide.