Figure 2.

NBP protects mitochondrial function and inhibits ROS production in PC12 cells exposed to H₂O₂.

(A) PC12 cells were treated with 200 µM H₂O₂ for 4 hours. The cells were pretreated with 40 µM NBP for 24 hours. Mitochondrial membrane potential was determined by evaluation of TMRE staining under a fluorescence microscope (100× magnification). (B) Detection of ROS by DCFH-DA staining in PC12 cells (100× magnification). ROS levels were increased after H₂O₂ exposure compared with controls, and NBP pretreatment inhibited the increase in ROS levels. The experiment was repeated three times. CTL: Control; DCFH-DA: 2,7-dichlorodi -hydrofluorescein diacet; NBP: Dl-3-n-butylphthalide; ROS: reactive oxygen species; TRME: N,N,N’,N’-tetra-methylethylenediamine.