Figure 6.

NBP inhibits H₂O₂-induced ECM degradation.

(A) RAW 264.7 cells were treated with 200 µM H₂O₂ and 40 µM NBP for 8 hours. NBP inhibited H₂O₂-induced ECM degradation in RAW 264.7 cells. Cells were plated on coverslips coated with FITC-gelatin (green) for 8 hours. F-actin was stained with phalloidin (red), and nuclei were stained with DAPI (blue) (100× magnifications). Black areas indicate gelatin degradation. (B) Mmp-2 and Mmp-9 mRNA levels were detected by real-time polymerase chain reaction. Data normalized by control group are presented as mean ± SD. The experiment was repeated three times. **P < 0.01, vs. control group (one-way analysis of variance followed by Bonferroni correction). CTL: Control; DAPI: 4′,6-diamidino-2-phenylindole; ECM: extracellular matrix; Mmp: matrix metalloproteinase; NBP: Dl-3-n-butylphthalide.