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. 2023 Mar 15;18(11):2429–2435. doi: 10.4103/1673-5374.371367

Figure 7.

Figure 7

LZ-3 decreases microglia phagocytosis and migration after ischemia or hypoxia.

(A) Representative images of immunofluorescence staining for NeuN (red, Alexa Fluor® 647) and Iba-1 (green, Alexa Fluor® 488) in the brain cortex. LZ-3 reduced the number of Iba-1- and NeuN-positive cells compared with Mod. Merged images show microglia phagocytosing neurons. Scale bars: 100 μm (left) and 10 μm (right). (B) The number of NeuN+ neurons phagocytosed by Iba-1+ microglia (30×) (n = 3). ##P < 0.01, vs. sham group; *P < 0.05, vs. model group. (C) Representative images of the cell migration assay performed after 4 hours of OGD. BV2 cells gradually migrated over the scratched area, and LZ-3 and upadacitinib were found to reduce the migration at 12 hours compared with the OGD group. Scale bar: 200 μm. (D) The migration rate of BV2 cells as shown in C (n = 3). ##P < 0.01, vs. control group; *P < 0.05, vs. OGD group. Data are expressed as mean ± SD and were analyzed by one-way analysis of variance followed by Tukey’s post hoc test. Con: Control group; DAPI: 4′,6-diamidino-2-phenylindole; Iba-1: ionized calcium binding adapter molecule-1; Mod: model group; NeuN: neuronal nuclear protein; OGD: oxygen-glucose deprivation.