FIG. 2.

Levels of trehalose and Tre6P synthase activity (A), Tps1 protein (B), and TPS1 mRNA (C) of wild-type H. polymorpha cells during heat shock and subsequent recovery. Cultures were grown at 27°C on YPD medium to early exponential phase, shifted to 47°C at time zero, and transferred back to 27°C after 120 min. (A) Black circles, trehalose levels; white circles, Tre6P synthase activities. Error bars indicate standard deviations. (B) Protein samples were extracted at the times indicated and subjected to immunoblot analysis of Tps1 protein levels (for further details see the legend for Fig. 1). (C) Total RNAs were extracted (14) at the times indicated, and equal amounts (10 μg) were probed as described in the legend for Fig. 1.