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. 2023 Mar 15;18(11):2535–2542. doi: 10.4103/1673-5374.371373

Figure 6.

Figure 6

HIS upregulates the expression of RvE1-ChemR23 axis in the spinal cord and increases RvE1 levels in the CSF but not the serum of CPIP mice.

(A) RvE1 levels in the spinal lumbar enlargement were significantly decreased after CPIP model establishment (n = 6 mice/group). ****P < 0.0001, vs. control group. (B) The mRNA levels of GPR37, ChemR23, and FPR2 in the bilateral lumbar enlargement of the spinal cord were decreased significantly in CPIP mice compared with control mice (n = 12 mice/group). ***P < 0.001 and ****P < 0.0001, vs. control group. (C, D) ChemR23 protein levels in the lumbar enlargement of the spinal cord were significantly decreased in CPIP mice compared with control mice (n = 12 mice/group). **P < 0.01, vs. control group. (E) HIS increased RvE1 levels in spinal lumbar enlargement of CPIP mice (n = 12 mice/group). *P < 0.05, vs. no exercise mice in the CPIP group. (F, G) HIS exercise increased RvE1 levels in the CSF (F) but not in the serum (G) in CPIP mice (n = 3 samples/group, n = 18 mice/group (F); n = 7 mice/group (G)). *P < 0.05, vs. no exercise mice in the CPIP group. (H) ChemR23 and FPR2 mRNA levels in the lumbar enlargement of the spinal cord were significantly increased in CPIP mice after HIS exercise (n = 6 mice/group). *P < 0.05 and ***P < 0.001, vs. no exercise mice in the CPIP group. (I, J) The ChemR23 protein level in the lumbar enlargement of the spinal cord of CPIP mice was significantly increased after HIS (n = 9 mice/group). **P < 0.01, vs. no exercise mice in the CPIP group. All data are presented as the mean ± SEM, and were analyzed by two-tailed unpaired Student’s t-test (A, B, D–H, J). Detailed statistical information is shown in Additional Table 2 (125.3KB, pdf) and Additional file 1 (136.8KB, pdf) . ChemR23: Chemerin receptor 23; Control: the ankles of mice were surrounded by a cut-O-ring; CPIP Exercised: mice were submitted to chronic postischemia pain and performed high-intensity swimming; CPIP Non-exercised: mice were submitted to chronic postischemia pain and were not performed swimming; CPIP: mice were submitted to chronic postischemia pain; CSF: cerebrospinal fluid; FPR2: formyl peptide receptor 2; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; GPR: G protein-coupled receptor; RvE1: Resolvin E1.