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. 2023 Mar 15;18(11):2474–2481. doi: 10.4103/1673-5374.371377

Figure 2.

Figure 2

Liproxstatin-1 regulates the major factors of the ferroptosis pathway after SCI.

(A) Timeline of the ferroptosis pathway analysis in acute SCI in rats. (B, D) Representative western blot image of the expressions of glutathione peroxidase 4 (GPX4) (B) and Acyl-CoA synthetase long-chain family member 4 (ACSL4; D) at 3 days after SCI. (C, E) Quantification of the expression of GPX4 (normalized to the Sham group) and ACSL4 (normalized to the SCI group) in spinal cord tissues in each group (n = 3). (F) Quantitative analysis of the fluorescence intensity of 4-hydroxynonenal (4-HNE) in spinal cord tissue (n = 3). (G) Quantitative analysis of the fluorescence intensity of malondialdehyde (MDA) in spinal cord tissue (n = 3). (H) The concentration of glutathione (GSH) in spinal cord tissue at 3 days after SCI (n = 6). (I) The concentration of iron in spinal cord tissue at 3 days after SCI (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Data were analyzed by one-way analysis of variance followed by Tukey’s post hoc test. Lip-1: Liproxstatin-1; SCI: spinal cord injury.