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. 2023 Mar 15;18(11):2474–2481. doi: 10.4103/1673-5374.371377

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Liproxstatin-1 regulates ferroptosis of vascular endothelial cells after SCI.

(A) Timeline of the experiments. (B, C) Representative confocal immunofluorescence images of Acyl-CoA synthetase long-chain family member 4 (ACSL4; green) (B), 15-lipoxygenase (15-LOX; green) (C), rat endothelium cell antigen-1 (RECA-1; red) and 4′,6-diamidino-2-phenylindole (DAPI; blue) in the epicenter. Scale bars: 200 µm and 20 µm (in magnified figures). Boxes represent the magnified region. (D, E) Quantitative analysis of the relative fluorescence intensity of ACSL4 (D) and 15-LOX (E) in RECA-1⁺ area (n = 3). *P < 0.05, **P < 0.01. Data were analyzed by one-way analysis of variance followed by Tukey’s post hoc test. Lip-1: Liproxstatin-1; SCI: spinal cord injury.