Table 1.
Primary antibody information
| Antibody | Host species | Source | Catalog number | RRID | Dilution |
|---|---|---|---|---|---|
| Anti-4 HNE | Mouse | Abcam, Cambridge, MA, USA | ab48506 | AB_867452 | 1: 25 (IF) |
| Anti-MDA | Mouse | Abcam | ab243066 | NA | 1:200 (IF) |
| Anti-RECA1 | Mouse | Abcam | ab9774 | AB_296613 | 1:200 (IF) |
| Anti-GPX4 | Rabbit | Abcam | ab125066 | AB_10973901 | 1:200 (IF)/ 1:5000 (WB) |
| Anti-ACSL4 | Rabbit | Abcam | ab155282 | AB_2714020 | 1:200 (IF)/ 1:10000 (WB) |
| Anti-15-LOX | Rabbit | Abcam | ab244205 | AB_2910599 | 1:50 (IF) |
| Anti-CD68 | Rabbit | Abcam | ab125212 | AB_10975465 | 1:500 (IF) |
| Anti-MPO | Rabbit | Abcam | ab208670 | AB_2864724 | 1:100 (IF) |
| Anti-NeuN | Mouse | Abcam | ab104224 | AB_10711040 | 1:200 (IF) |
| Anti-GFAP | Rabbit | Abcam | ab7260 | AB_305808 | 1:200 (IF) |
| Anti-ZO-1 | Rabbit | Proteintech, Rosemont, IL, USA | 21773-1-AP | AB_10733242 | 1:200 (IF) |
| Anti-beta-actin | Mouse | Proteintech | 66009-1-ig | AB_2687938 | 1:1000 (IF) |
| Anti-GAPDH | Mouse | Santa Cruz, CA, USA | SC32233 | AB_627679 | 1:500(WB) |
4-Hydroxynonenal (4-HNE) and malondialdehyde (MDA) are the lipid peroxidation markers. Rat endothelial cell antigen-1 (RECA-1) is the marker of rat endothelial cells. Glutathione peroxidase 4 (GPX4), acyl-CoA synthetase long-chain family member 4 (ACSL4) and 15-lipoxygenase (15-LOX) are both ferroptosis markers. CD68 is the marker of activated macrophages and microglia. Myeloperoxidase (MPO) is the marker of neutrophil granulocyte. Neuronal nuclear antigen (NeuN) is a marker of neurons and glial fibrillary acidic protein (GFAP) represents astrogliosis. Zonula occludens-1 (ZO-1) is a representative tight junction protein.