Figure 1.

The expression levels of m6A writers, readers and erasers after SNI.

(A) Flow chart of the experimental analysis. (B) von Frey test after SNI at the 3^rd, 7^th and 14^th days. (C) Quantitative RT-PCR showing the upregulated levels of m6A readers (Ythdf2 and Ythdf3) at the indicated time points after SNI. (D–F) Quantitative RT-PCR showing the expression levels of m6A erasers (D), readers (E) and writers (F) at the indicated time points after SNI. Data are expressed as mean ± SD. *P < 0.05, **P < 0.01 (two-way analysis of variance analysis of variance (B) or one-way ANOVA analysis of variance followed by Dunnett’s multiple comparisons test (C–E). Three mice were used for qRT-qPCR and a total of 14 replicates were performed for all samples for each enzyme. MeRIP-Seq: Methylated RNA immunoprecipitation sequencing; qRT-PCR: quantitative reverse transcription-polymerase chain reaction; RNA-Seq: RNA sequencing; SNI: spared nerve injury.