Figure 1: Coating the microelectrode array (MEA) prior to use.
(A) The MEA is composed of a small plastic dish with a grid array of 64 microelectrodes in the center (as shown in the panel A1) and four reference electrodes around the periphery in a square pattern. (B) Addition of 1 mL of PEI buffer to coat the MEA. (C) Covering the MEA dish with thermoplastic film for incubation overnight at room temperature. (D) Aspirating the PEI buffer from the MEA dish, which is followed by at least four rinses with distilled water. (E) Storing the coated MEA probe under ultrapure water to prevent it from drying out.