Figure 3.

Assessment of the morphological transition of self-assembled Janus dendrimers via 37 °C annealing in the absence of ethanol. (a) D_h profiles as determined by DLS in the presence (+EtOH) and absence (−EtOH) of ethanol. (b) Relative change of D_h (%), PDI (%), and derived count rates (%) as compared with the sample with ethanol as measured by DLS. (c,d) Relative change of D_h (%), derived count rates (%), PDI (%) as measured by DLS (c), and D_h (%) and particle concentration (%) as measured by NTA (d) of the sample without ethanol as a function of annealing time at 37 °C. Characterization was performed on three different batches of samples (n = 3). (e–g) Cryo-TEM images of self-assemblies after removing EtOH (e) and assemblies annealed from 37 °C after being equilibrated for 0.5 (f) and 1 h (g). The insets display frequency histograms illustrating the distribution of the bilayer thickness. Each histogram was fitted with the Gaussian distribution, as depicted by the solid lines. (h) Quantitative measurement of assemblies obtained from direct injection (e) and post-treatment at 37 °C (f,g). For each condition, images from different areas and different batches of samples were taken and counted to minimize the error (n > 500 particles).