Figure 5.
Assessment of the morphological transition mechanism at different temperatures. Snapshots of cryo-TEM images of self-assemblies: (a) at room temperature (R.T. I), (b) 0, 1, and 4 min at 37 °C, and (c) 1 and 4 min at 43 °C. Scale bars are 100 nm. Tubular vesicles with multiple stacked bilayers are highlighted by arrows in (b) for 1 min. Initial OLVs with stacked bilayers, OLVs with discernible bilayers, and immature MLVs are highlighted by white, yellow, and blue arrows in (c) 1 min, respectively.