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. 2023 Jul 7;14:1204889. doi: 10.3389/fpls.2023.1204889

Figure 1.

Figure 1

Microtubule binding protein END BINDING1 (EB1-mCherry) localizes to microtubule plus-ends and extensively co-localizes with YFP-TUBULIN during mitosis. Microtubules (false-colored green) from the abaxial side of maize leaves in regions with symmetrically dividing cells were imaged with YFP-TUBULIN and EB1 is false-colored magenta. (A) EB1-mCherry labels the plus-end of growing microtubules. (B) Timelapse of a cell in prophase. Arrowheads mark the EB1-mCherry protein. Zoom images of a prophase cell to visualize EB1. Arrowheads point at EB1-mCherry as the microtubule grows in the timelapse. Background subtraction was used in FIJI. Scale bars are 10µm. (C) Kymograph of EB1-mCherry protein tracking plus-end of microtubule. EB1-mCherry disappears followed by microtubule shrinkage (arrowhead). (D) Microtubule growth and catastrophe speed (µm/min) in wild-type maize leaf epidermal cells from 4 plants in interphase (n = 29 cells), prophase and preprophase (n = 2 cells) and telophase cells (n = 2 cells). (E) EB1 particle speed (µm/min) in wild-type maize leaf epidermal cells from 2 plants in interphase (n = 7 cells) and pre-prophase and prophase cells (n = 10 cells). P-values * < 0.05, ** < 0.01, **** < 0.0001 by Mann-Whitney U test.