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. 2023 Jul 7;14:1204889. doi: 10.3389/fpls.2023.1204889

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Copyright © 2023 Allsman, Bellinger, Huang, Duong, Contreras, Romero, Verboonen, Sidhu, Zhang, Steinkraus, Uyehara, Martinez, Sinclair, Soriano, Diep, Byrd V., Noriega, Drakakaki, Sylvester and Rasmussen

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Chromosome marker HISTONE1.1-YFP and nuclear envelope marker RANGAP1-YFP with CFP-TUBULIN during the cell cycle. Microtubules from the abaxial side of maize leaves in regions with symmetrically dividing cells were imaged with CFP-TUBULIN (top row). In the merged images, microtubules are colored green, while HISTONE1.1-YFP or RANGAP1-YFP are colored magenta. (A) Chromosomes are labeled with HISTONE1.1-YFP (middle row). (B) RANGAP1-YFP labels the cell periphery, the nuclear envelope during interphase and preprophase/prophase, and localizes close to the PPB during prophase/preprophase. During mitosis, RANGAP1-YFP evenly labels all microtubule structures but is not at the division site. Preprophase/prophase RANGAP1-YFP and CFP-TUBULIN images are maximum-projections of Z stacks covering 7.5 microns to more clearly illustrate RANGAP1-YFP localization near the PPB. Scale bar is 10µm, all images are the same size.