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. 2023 Jul 7;14:1204889. doi: 10.3389/fpls.2023.1204889

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Copyright © 2023 Allsman, Bellinger, Huang, Duong, Contreras, Romero, Verboonen, Sidhu, Zhang, Steinkraus, Uyehara, Martinez, Sinclair, Soriano, Diep, Byrd V., Noriega, Drakakaki, Sylvester and Rasmussen

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ER membrane marker GLOSSY8-mRFP and ER lumen marker PDI1- YFP with CFP-TUBULIN. Microtubules from the abaxial side of maize leaves in regions with symmetrically dividing cells were imaged with CFP-TUBULIN (top row). Microtubules are labeled green, while GLOSSY8-mRFP and PDI1-YFP are labeled magenta, in the merged photos (bottom row). (A) Endoplasmic reticulum membrane is labeled with GLOSSY8-mRFP. GLOSSY8-mRFP co-localizes with distal spindle regions during metaphase and anaphase. GLOSSY8-mRFP accumulates at the cell plate during telophase. (B) PDI1-YFP localization labels the endoplasmic reticulum lumen. PDI1-YFP localizes to distal spindle regions during metaphase and anaphase. Accumulation of PDI1-YFP is present near or in the cell plate and in the phragmoplast during telophase. Scale bar is 10µm.