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. 2023 Jul 7;14:1204889. doi: 10.3389/fpls.2023.1204889

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Copyright © 2023 Allsman, Bellinger, Huang, Duong, Contreras, Romero, Verboonen, Sidhu, Zhang, Steinkraus, Uyehara, Martinez, Sinclair, Soriano, Diep, Byrd V., Noriega, Drakakaki, Sylvester and Rasmussen

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Plasma membrane markers PIN1 (PIN1-YFP) and PIP2 (PIP2-CFP). Microtubules were imaged with CFP-TUBULIN (left panels) and YFP-TUBULIN (right panels) in the top row. PIN1-YFP was imaged in the early fringe within the ligule and PIP2-CFP was imaged from the abaxial side of maize leaves in regions with symmetrically dividing cells in the middle row. Microtubules are labeled green and markers in magenta in the merged photos (bottom row). (A) PIN1-YFP localizes to the plasma membrane during interphase and all stages of mitosis. PIN1-YFP accumulates at the cell plate during telophase. (B) PIP2-CFP accumulates at the plasma membrane during interphase and all stages of mitosis in dividing epidermal tissue, and accumulates in the cell plate. Scale bars are 10µm.