Skip to main content
. 2023 Jul 7;14:1204889. doi: 10.3389/fpls.2023.1204889

Figure 6.

Figure 6

Effect of CIPC on the accumulation of YFP-KNOLLE, RAB11D-YFP, and callose to the cell plate. Microtubules imaged with the CFP-TUBULIN are labeled in green, with YFP-KNOLLE or RAB11D-YFP labeled in magenta, in the top and middle panels from the abaxial side of maize leaves in regions with symmetrically dividing cells. (A) Effect of 0.02% DMSO treatment on the accumulation of YFP-KNOLLE, RAB11D-YFP, and callose to the cell plate. (B) Effect of 2µM CIPC treatment on the accumulation of YFP-KNOLLE, RAB11D-YFP, and callose to the cell plate. (C) Percentage of cell plate morphology seen after callose staining on samples treated with either 0.02% DMSO or 2µM CIPC. DMSO: Stub = 0%, Split = 2%, Normal= 95%; CIPC: Stub = 18%, Split = 34%, Normal = 48%. Number of plants = 2, CIPC number of cells = 61, DMSO number of cells = 59. Scale bar is 10µm. For callose staining both YFP-KNOLLE and RAB11D-YFP number of plants = 3. In the YFP-KNOLLE plants cells in telophase n = 89 in the CIPC treatment, while for the DMSO treatment cells in telophase n = 37 cells. In the RAB11D-YFP plants, a total of telophase cells n = 36 were seen when treated with CIPC, while telophase n = 29 cells in the DMSO treatment. **p ≤ 0.01 unpaired t-test.