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. 2023 Jul 7;10:1206279. doi: 10.3389/fcvm.2023.1206279

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© 2023 Desgres, Lima Correa, Petrusca, Autret, Pezzana, Marigny, Guillas, Bellamy, Vilar, Perier, Dingli, Loew, Humbert, Larghero, Churlaud, Renault, Croisille, Hagège, Silvestre and Menasché.

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EV-CPC characterization. (A) Cryo-TEM resolution of EV-CPC lipid bilayer membranes. Scale bar = 100 μm. (B) Size distribution of isolated particles by NTA analysis measured on 5 different preparations of EV-CPC. (C) FACS detection of tetraspanin expression on EV-CPC by MACSPlex analysis compared to a negative control, i.e., the CD31 endothelial marker (in technical duplicates; median +/− IQR). (D) Extract from the absolute quantification of proteomic analysis. SD: standard deviation; NTA: Nanoparticle Tracking Analysis; Cryo-TEM: immuno-gold labelling and cryo-transmission electron microscopy; CD: cluster of differentiation; MW: molecular weight.