Figure 2.

Monocyte-endothelial cell interactions and vascular sprouting occurring after hepatectomy. (A) Images of vessels from liver sections identified by staining for von Willebrand factor (VWF, in red) and recruited monocytes by staining for CD14 (in green). Nuclei were stained by DAPI (in blue). Initial contacts of recruited monocytes (in yellow) take place in portal areas. (B) Amplification of multiphoton images (vessels in green and yellow, macrophages in red), visualized vascular buds (white circles) surrounded by spread recruited macrophages 16 hours after hepatectomy. Images reprinted with permission from “Melgar-Lesmes, P.; et al. Monocyte-endothelial cell interactions in the regulation of vascular sprouting and liver regeneration in mouse. J Hepatol. 2015; 63 4):917-25. doi: 10.1016/j.jhep.2015.05.011”.