. 2023 Jul 21;24:465. doi: 10.1186/s13063-023-07477-9

Table 4.

Antigens and assays for primary and secondary immunogenicity outcomes

Antigen	Assay	Volume of serum required (µl)
Diphtheria toxin, tetanus toxin, pertussis (pertussis toxin, pertactin, filamentous hemagglutinin), Hib-PRP, hepatitis B surface antigen, polio types 1–3, pneumococcal antigen (all 10 serotypes)	Multiplexed immunoassay	150
Japanese encephalitis virus	Enzyme-linked immunosorbent assay	100
Japanese encephalitis virus	Plaque reduction neutralisation titres/indirect fluorescent antibody assay	50
Yellow fever virus	Enzyme-linked immunosorbent assay	100
Yellow fever virus	Plaque reduction neutralisation titres	30–80
Typhoid Vi antigen	Enzyme-linked immunosorbent assay (IgG and IgA)	60–100