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Fan inhibits PEDV replication by inhibiting autophagic degradation. (A) IPEC-J2cells were treated with 10 μM fangchinoline, 20 μM chloroquine, and 0.2 μM bafilomycin A1 for 1 h. At 24 h after PEDV (MOI = 0.1) infection, cell lysates were collected for Western blotting as indicated. (B) LC3, (C) P62, and (D) N protein of PEDV were quantitated. CQ, chloroquine; Baf A1, bafilomycin A1. *P < 0.05, ***P < 0.001, and ****P < 0.0001 indicate significant differences vs. the control group.
