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. Author manuscript; available in PMC: 2023 Dec 23.
Published in final edited form as: Sci Immunol. 2023 Jun 16;8(84):eade7652. doi: 10.1126/sciimmunol.ade7652

Fig. 4. Multiple VDACs participate in NLRP3 inflammasome activation.

Fig. 4.

(A) Immunoblot of VDAC1, VDAC3, IL-1β, and β-tubulin from LPS-primed BMDM in which VDAC1 and/or VDAC3 are knock-downed using gRNAs (cr-VDAC1, cr-VDAC3, Ctl = control/no gRNA). (B) Combined quantitative analysis of pro-IL-1β protein expression across independent experiments (n = 3). (C) Secretion of TNF-α into the culture supernatant was measured by ELISA (n = 9 replicates across 3 experiments). (D) IL-1β secretion was measured by ELISA in culture supernatants of LPS-primed cells with ATP (n = 11 replicates across 3 experiments), HK-TAT (n = 6 replicates across 2 experiments), pdA:dT or infection with S. Typhimurium (n = 12 replicates across 3 experiments). (E) Relative mRNA expression of VDAC1, VDAC2, and VDAC3 was measured by RT-qPCR in BMDM in which VDACs were knock-downed as indicated (n = 3 biological replicates). (F) IL-1β secretion was measured by ELISA from primed BMDM with the indicated VDACs knocked down and induced with ATP (n = 6). (G) TNF-α secretion in primed BMDM in which the indicated VDACs are knocked down (n = 6). Data are presented as means ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (one-way ANOVA, Tukey’s post hoc test).