TABLE 2.
Transfer frequencies of pSB24.2 Clt+ derivatives from S. lividans TK23.42a
| Plasmid
|
Avg no. of:
|
Transfer frequencyb (%) | |||
|---|---|---|---|---|---|
| Donor (relevant genotype) | Recipient | Donors | Recipients | Transconjugants | |
| pGSP263 (clt+ [pIJ101]) | pHYG1 | 3.8 × 105 | 2.1 × 107 | 1.1 × 105 | 29 |
| pGSP149 (clt) | pHYG1 | 9.0 × 104 | 1.1 × 107 | 7.2 × 102 | 0.80 |
| pSB24.202 (clt+ [pSB24.2]) | pHYG1 | 9.7 × 105 | 3.9 × 107 | 2.7 × 105 | 28 |
| pGSP313 (clt+ [pSB24.2]) | pHYG1 | 2.6 × 105 | 2.6 × 107 | 6.3 × 104 | 24 |
a
Matings were performed and quantified as described previously (17). Numbers represent the averages obtained from four independent mating assays.
b
Transfer frequency is calculated as the ratio of transconjugants to donors expressed as a percentage.