FIG. 2.

Physical map of Tcm, actinorhodin (ACT), and DNR PKS gene clusters. The actI-ORF1, actI-ORF2, tcmK, tcmL, dpsA, and dpsB genes encode the KS subunits; the actI-ORF3, tcmM, and dpsG genes encode the ACPs; the actVII, actIV, tcmJ, tcmN, dpsF, and dpsY genes encode the polyketide cyclases; and the actIII and dpsE genes encode ketoreductases. The dnrG gene specifies an anthraquinol oxygenase, and the dpsC and dpsD genes encode KS III-like and acyltransferase enzymes, respectively. ORF, open reading frame; //, indication that the genes are separated by several kilobases.