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Cell-free synthesis of polyketides
| Plasmids | Proteins present | Metabolite from [1-14C]malonyl CoA |
|---|---|---|
| pWHM80 | DnrG and DpsABEFG | —a |
| pWHM80 | DnrG and DpsABEFG + purified TcmN | Tcm F2 |
| pWHM1011 | TcmJ and DpsABCDGEF | UWM5 |
| pWHM1010 + pWHM1015 | TcmJ and DpsABCDG + DpsEF | UWM5 |
| pWHM1080 + pWHM1014 | DnrG and DpsABEFG + DpsCD | UWM5 |
| pWHM80 | DnrG and DpsABEFG + purified DpsC | UWM5 |
—, small amount of SEK43 was produced.
