FIG. 2.

Influence of overexpressed α-256 on LuxR and LuxRΔN activities in E. coli. Each value represents the average of two independent experiments, each done in triplicate. The marker bars indicate the range. Cultures containing plasmids expressing the lux operon, either wild-type (WT) RpoA or α-256 RpoA, and either LuxR, LuxRΔN, or no LuxR, as indicated, were grown to an optical density at 600 nm of 0.5, and luciferase activity was measured. The luciferase activity in cells containing the wild-type RpoA expression plasmid and the LuxR expression plasmid is given as 100%.