TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| E. coli strains | ||
| DH5α | supE44 ΔlacU169 (φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | 14 |
| JM109 | recA1 supE44 endA1 hsdR17 gyrA96 relA1 thiΔ(lac-proAB) F′ (traD36 proAB+ lacIqlacZΔM15) | 25 |
| Plasmids | ||
| pSC300 | luxR in pKK223-3, Apr | 4 |
| pSC156 | luxR with deletion of sequences encoding N-terminal amino acids 2–156 of LuxR in pKK223-3, Apr | 3 |
| pSUP102 | pACYC184, RP4, Cmr Tcr Mob+ | 19 |
| pAMS121 | 1-kb BamHI fragment with ptac-luxR from pSC300 in BamHI site of pSUP102, Cmr Tcs Mob+ | This study |
| pAMS122 | 0.6-kb BamHI fragment with ptac-ΔluxR from pSC156 in BamHI site of pSUP102, Cmr Tcs Mob+ | This study |
| pJE202 | 9-kb SalI fragment of V. fischeri DNA (luxR luxICDABEG) in pBR322, Apr | 10 |
| pJRD215 | Broad-host-range RSF1010-based cosmid vector, Knr Smr Mob+ | 5 |
| pAMS129 | 8-kb XbaI-SalI fragment with luxR′ luxICDABEG from pJE202 in XbaI-SalI sites of pJRD215, Knr Smr Mob+ | This study |
| pLAX185 | rpoA in pINIIIA1, Apr | 15 |
| pLAD256 | rpoA with deletion of sequences encoding C-terminal 73 amino acids of RpoA in pINIIIA1, Apr | 15 |
| pAMS103 | lux intergenic region in pUC19, Apr | 23 |
| pAMS1300 | luxI promoter in pMP7, Apr | 23 |