Fig. 3.

Cytotoxicity and cell viability of NK-exos^IL−15/21 in HCC cells. Hep3B cells were co-treated with NK-exos, NK-exos^IL−15, NK-exos^IL−21, and NK-exos^IL−15/21 (each, 50 μg) for 24 h. (A) Staining of Hep3B cells with a staining solution containing 0.5 mM calcein-AM and 4 mM EthD-1 for 30 min. Live (green) and dead (red) cells were detected by live/dead assay and visualized by confocal microscopy. Scale bars: 200 μm. (B) Quantification of live and dead cells. (C) Measurement of the cell viability of Hep3B cells by MTT assay. All data are presented as the mean ± SD (n = 3). *p < 0.05, **p < 0.01, and ***p < 0.001 vs. untreated Hep3B cells. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)