Fig. 4.

Apoptotic activity of NK-exos^IL−15/21 in HCC cells. (A) Co-culture of Hep3B cells with NK-exos, NK-exos^IL−15, NK-exos^IL−21, and NK-exos^IL−15/21 (each, 50 μg) for 24 h. The cells were stained with Annexin V-FITC/PI and analyzed by flow cytometry. Q1: live cells (Annexin V−/PI−); Q2: early apoptotic cells (Annexin V+/PI−); Q3: late apoptotic cells (Annexin V+/PI+); Q4: necrotic cells (Annexin V−/PI+). (B) Quantification of necrotic, apoptotic, and live cells. All data are presented as the mean ± SD (n = 3). **p < 0.01, ***p < 0.001, and N.S. (no significance) vs. untreated Hep3B cells.