Fig. 4.

Comparison of NET quantification by hand counting semi-automated quantification and NETQUANT.

A: Cell numbers for Image set 1 determined by all three methods, showing significantly fewer cell counts by NETQUANT in the majority of the images. B: NET formation determined by all methods revealed a significant difference between hand counting and NETQUANT as well as the semi-automated method. Statistical analysis was performed by Two-Way ANOVA with Dunnett correction for multiple comparison. Semi-automated and NETQUANT values were compared with hand counting. Data are given as mean ± SD. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).