Figure 1.
In vitro hairy root induction and RUBY expression in soybean. (A) Emergence of white calli at the basal end of cultured cotyledons (red arrows) (bar= 5 mm). (B) The emergence of the first hairy roots on the surface of elongated hypocotyls (red arrows) (bar= 5 mm). (C) The first signs of red hairy roots on the surface of induced calli (red arrow) (bar= 5 mm). (D) Induced red hairy roots from accidentally broken cotyledons (bar= 5 mm). (E) Elongation of red hairy roots in root induction medium (bar= 3 cm). (F) Shoot induction from cotyledons with red hairy roots (bar= 3 cm). (G) Composite soybean plantlet with a large mass of red hairy roots expressing RUBY (bar= 3 cm). (H) Acclimatized composite soybean plant (bar= 3 cm).
