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. 2023 Jul 7;14:1207762. doi: 10.3389/fpls.2023.1207762

Figure 5.

Figure 5

Molecular analysis of putative transgenic red hairy roots for the presence of the bar gene and expression of the RUBY gene. (A) PCR detection of the bar gene in transgenic hairy roots. M: DNA marker (100 bp), 1-12: red putatively transgenic hairy roots, P: 35S_Ω_RUBY plasmid as a positive control (+), C: white hairy roots as a negative control, H2O: negative control without template. (B) Expression of the RUBY reporter gene in transgenic hairy roots normalized relative to ubiquitin (Gma.441.1.S1_at) and actin (GmaAffx.90181.1.A1_at) genes using the 2-△△CT method.