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. 2023 Jul 7;14:1207762. doi: 10.3389/fpls.2023.1207762

Table 1.

Mean percentage of hairy root induction in soybean line 20SS01 via in vitro transformation.

Strain Cell
Density
(OD600)
Inoculation duration (min)
10 20 30
R1000 0.30 40.00 ± 2.98e 73.33 ± 2.98b 100 ± 0.00a
0.50 53.33 ± 2.98d 60.00 ± 2.98cd 66.66 ± 2.98bc
0.80 0.00 ± 0.00h 6.66 ± 2.98gh 20.00 ± 0.00fg
A4 0.30 0.00 ± 0.00h 0.00 ± 0.00h 6.66 ± 2.98gh
0.50 13.33 ± 2.98fh 20.00 ± 2.98fg 40 ± 0.00e
0.80 6.66 ± 2.98gh 13.33 ± 2.98fh 6.66 ± 2.98gh
ARqual 0.30 0.00 ± 0.00h 0.00 ± 0.00h 20.00 ± 0.00fg
0.50 20.00 ± 0.00fg 26.66 ± 2.98f 60.00 ± 0.00cd
0.80 20.00 ± 0.00fg 26.66 ± 2.98f 40.00 ± 0.00e
K599 0.30 0.00 ± 0.00h 6.66 ± 2.98gh 20.00 ± 0.00fg
0.50 13.33 ± 2.98fh 66.66 ± 2.98bc 100 ± 0.00a
0.80 6.66 ± 2.98gh 20.00 ± 0.00fg 26.66 ± 2.98f

Values represent the mean ± standard error from three replications. Values followed by the same letter are not significantly different at P< 0.05.

Three parameters (strain, cell density and duration of inoculation) were explored in a factorial experiment where each of 36 treatments (five cotyledons/treatment) was replicated three times.