Table 1.
Mean percentage of hairy root induction in soybean line 20SS01 via in vitro transformation.
Strain | Cell Density (OD600) |
Inoculation duration (min) | ||
---|---|---|---|---|
10 | 20 | 30 | ||
R1000 | 0.30 | 40.00 ± 2.98e | 73.33 ± 2.98b | 100 ± 0.00a |
0.50 | 53.33 ± 2.98d | 60.00 ± 2.98cd | 66.66 ± 2.98bc | |
0.80 | 0.00 ± 0.00h | 6.66 ± 2.98gh | 20.00 ± 0.00fg | |
A4 | 0.30 | 0.00 ± 0.00h | 0.00 ± 0.00h | 6.66 ± 2.98gh |
0.50 | 13.33 ± 2.98fh | 20.00 ± 2.98fg | 40 ± 0.00e | |
0.80 | 6.66 ± 2.98gh | 13.33 ± 2.98fh | 6.66 ± 2.98gh | |
ARqual | 0.30 | 0.00 ± 0.00h | 0.00 ± 0.00h | 20.00 ± 0.00fg |
0.50 | 20.00 ± 0.00fg | 26.66 ± 2.98f | 60.00 ± 0.00cd | |
0.80 | 20.00 ± 0.00fg | 26.66 ± 2.98f | 40.00 ± 0.00e | |
K599 | 0.30 | 0.00 ± 0.00h | 6.66 ± 2.98gh | 20.00 ± 0.00fg |
0.50 | 13.33 ± 2.98fh | 66.66 ± 2.98bc | 100 ± 0.00a | |
0.80 | 6.66 ± 2.98gh | 20.00 ± 0.00fg | 26.66 ± 2.98f |
Values represent the mean ± standard error from three replications. Values followed by the same letter are not significantly different at P< 0.05.
Three parameters (strain, cell density and duration of inoculation) were explored in a factorial experiment where each of 36 treatments (five cotyledons/treatment) was replicated three times.