. 2023 Jul 7;14:1207762. doi: 10.3389/fpls.2023.1207762

Table 1.

Mean percentage of hairy root induction in soybean line 20SS01 via in vitro transformation.

Strain	Cell Density (OD₆₀₀)	Inoculation duration (min)
Strain	Cell Density (OD₆₀₀)	10	20	30
R1000	0.30	40.00 ± 2.98^e	73.33 ± 2.98^b	100 ± 0.00^a
	0.50	53.33 ± 2.98^d	60.00 ± 2.98^cd	66.66 ± 2.98^bc
	0.80	0.00 ± 0.00^h	6.66 ± 2.98^gh	20.00 ± 0.00^fg
A4	0.30	0.00 ± 0.00^h	0.00 ± 0.00^h	6.66 ± 2.98^gh
	0.50	13.33 ± 2.98^fh	20.00 ± 2.98^fg	40 ± 0.00^e
	0.80	6.66 ± 2.98^gh	13.33 ± 2.98^fh	6.66 ± 2.98^gh
ARqual	0.30	0.00 ± 0.00^h	0.00 ± 0.00^h	20.00 ± 0.00^fg
	0.50	20.00 ± 0.00^fg	26.66 ± 2.98^f	60.00 ± 0.00^cd
	0.80	20.00 ± 0.00^fg	26.66 ± 2.98^f	40.00 ± 0.00^e
K599	0.30	0.00 ± 0.00^h	6.66 ± 2.98^gh	20.00 ± 0.00^fg
	0.50	13.33 ± 2.98^fh	66.66 ± 2.98^bc	100 ± 0.00^a
	0.80	6.66 ± 2.98^gh	20.00 ± 0.00^fg	26.66 ± 2.98^f

Values represent the mean ± standard error from three replications. Values followed by the same letter are not significantly different at P< 0.05.

Three parameters (strain, cell density and duration of inoculation) were explored in a factorial experiment where each of 36 treatments (five cotyledons/treatment) was replicated three times.