. 2023 Jul 7;14:1207762. doi: 10.3389/fpls.2023.1207762

Table 2.

Mean percentage of transformation efficiency in 20SS01 genotype via in vitro transformation.

Strain	Cell density (OD₆₀₀)	Inoculation duration (min)
Strain	Cell density (OD₆₀₀)	10	20	30
R1000	0.30	0.00 ± 0.00^e	20.00 ± 5.16^cd	66.66 ± 2.98^a
	0.50	13.33 ± 2.98^de	0.00 ± 0.00^f	26.66 ± 2.98^c
	0.80	0.00 ± 0.00^f	0.00 ± 0.00^f	0.00 ± 0.00^f
A4	0.30	0.00 ± 0.00^f	0.00 ± 0.00^f	0.00 ± 0.00^f
	0.50	0.00 ± 0.00^f	0.00 ± 0.00^f	0.00 ± 0.00^f
	0.80	0.00 ± 0.00^f	0.00 ± 0.00^f	0.00 ± 0.00^f
ARqual	0.30	0.00 ± 0.00^f	0.00 ± 0.00^f	0.00 ± 0.00^f
	0.50	0.00 ± 0.00^f	0.00 ± 0.00^f	13.33 ± 2.98^de
	0.80	0.00 ± 0.00^f	0.00 ± 0.00^f	6.66 ± 2.98^ef
K599	0.30	0.00 ± 0.00^f	0.00 ± 0.00^f	0.00 ± 0.00^f
	0.50	0.00 ± 0.00^f	13.33 ± 2.98^ef	53.33 ± 2.98^b
	0.80	0.00 ± 0.00^f	0.00 ± 0.00^f	0.00 ± 0.00^f

Values represent the mean ± standard error from three replications. Values followed by the same letter are not significantly different at P< 0.05.

Three parameters (strain, cell density and duration of inoculation) were explored in a factorial experiment where each of 36 treatments (five cotyledons/treatment) was replicated three times.