Table 3.
Mean percentage of hairy root induction in soybean via in vitro transformation.
| Plant genotype | Strain | Age of explants | ||
|---|---|---|---|---|
| 5-d | 7-d | 10-d | ||
| Williams | R1000 | 46.66 ± 2.98def | 73.33 ± 2.98bc | 60.00 ± 0.00cd |
| A4 | 0.00 ± 0.00k | 6.66 ± 2.98jk | 0.00 ± 0.00k | |
| ARqual | 0.00 ± 0.00k | 13.33 ± 2.98ijk | 6.66 ± 2.98jk | |
| K599 | 20.00 ± 0.00hij | 100.00 ± 0.00a | 73.33 ± 2.98bc | |
| Jack | R1000 | 13.33 ± 2.98ijk | 46.66 ± 2.98def | 26.66 ± 2.98ghi |
| A4 | 0.00 ± 0.00k | 13.33 ± 2.98ijk | 6.66 ± 2.98jk | |
| ARqual | 0.00 ± 0.00k | 40.00 ± 0.00efg | 13.33 ± 2.98ijk | |
| K599 | 13.33 ± 2.98ijk | 60.00 ± 0.00cd | 26.66 ± 2.98ghi | |
| Bert | R1000 | 33.33 ± 2.98fgh | 100.00 ± 0.00a | 73.33 ± 2.98bc |
| A4 | 0.00 ± 0.00k | 13.33 ± 2.98ijk | 6.66 ± 2.98jk | |
| ARqual | 0.00 ± 0.00k | 33.33 ± 2.98fgh | 6.66 ± 2.98jk | |
| K599 | 13.33 ± 2.98ijk | 80.00 ± 0.00b | 53.33 ± 2.98de | |
| 20SS01 | R1000 | 26.66 ± 2.98ghi | 100.00 ± 0.00a | 53.33 ± 2.98de |
| A4 | 0.00 ± 0.00k | 40.00 ± 0.00efg | 26.66 ± 2.98ghi | |
| ARqual | 0.00 ± 0.00k | 60.00 ± 0.00cd | 20.00 ± 5.16hij | |
| K599 | 6.66 ± 2.98jk | 100.00 ± 0.00a | 26.66 ± 2.98ghi | |
Values represent the mean ± standard error from three replications. Values followed by the same letter are not significantly different at P< 0.05.
Three parameters (strain, plant genotype and explant age) were explored in a factorial experiment where each of 48 treatments (five cotyledons/treatment) was replicated three times.