Table 8.
The results of optimization process for A. rhizogenes-mediated hairy root transformation of soybean in in vitro and ex vitro experiments.
| Experiment | Fitness function | Optimal level of input variables | Predicted fitness function value |
||||||
|---|---|---|---|---|---|---|---|---|---|
| A. rhizogenes strain | A. rhizogenes cell density (OD600) | A. rhizogenes inoculation duration (min) | Plant genotype | Age of explants (d) | Concentrations of acetosyringone (µmol/L) | Root induction media | |||
| In vitro | Hairy root induction | R1000 | 0.38 | 28.43 | 20SS01 | 6.89 | 93.83 | ¼ MS | 99.54 |
| RUBY gene transformation | R1000 | 0.32 | 29.00 | Bert | 7.00 | 126.74 | ½ B5 | 78.96 | |
| Ex vitro | Hairy root induction | R1000 | – | – | 20SS01 | 8.45 | – | – | 99.51 |
| RUBY gene transformation | R1000 | – | – | Bert | 8.66 | – | – | 59.28 | |