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. 2023 Jun 13;22(13):1529–1543. doi: 10.1080/15384101.2023.2215081

Figure 4.

Figure 4.

The effect of exercise-induced myocardial hypertrophy preconditioning on Nrf2 signaling pathway. (a,b) shows Qrt-PCR and western blot analysis for detecting Nrf2 mRNA and protein levels in cardiac fibroblasts, respectively. GAPDH was used as the housekeeping gene for the RT-qPCR experiments. (c, d) shows the Qrt-PCR and western blot analysis for detecting Nrf2 mRNA and protein levels, respectively, in cardiac tissues of an animal model. (e) indicates the Qrt-PCR analysis of HO-1, NQO-1, and GCLC mRNA levels in cardiac fibroblasts with GAPDH used as the control. (f) shows western blot analysis of HO-1, NQO-1, and GCLC protein levels in cardiac fibroblasts. ***P < 0.01, ##P < 0.05 vs. control, *P < 0.01 vs. NE. Data were presented as mean±SD from three independent experiments.