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. 2023 Jun 13;22(13):1529–1543. doi: 10.1080/15384101.2023.2215081

Figure 5.

Figure 5.

Effects of S100A8/9 on Nrf2 protein in fibroblasts. (a) Qrt-PCR analysis was performed to determine the expression of S100A8/9 using GAPDH as the housekeeping gene in the control, NE, and Pre + NE groups. (b and c) Western blot analysis of Nrf2 protein in basal fibroblasts, S100A8/9 overexpressing fibroblasts, and S100A8/9 silenced fibroblasts. The data indicate a significant increase in Nrf2 protein expression in S100A8/9 silenced fibroblasts compared to the control (***P < 0.001, ##P < 0.01 vs. control, *P < 0.01 vs. NE). All data presented are the mean±SD from three independent experiments.