Figure 7.

The impact of preconditioning and silencing of Nrf2 on the response of fibroblasts to oxidative stress induced by NE stimulation. The panel (a) shows representative fluorescence images of fibroblasts stained with DCFDA (2´,7´-dichlorofluorescein diacetate) for detecting ROS levels in four groups: control, NE, Pre + NE, and Pre + NE + Si-Nrf2. B the levels of SOD and GPx activities in these groups were measured using Superoxide Dismutase Activity Assay Kit and Glutathione Peroxidase Assay Kit, respectively. The control group served as a baseline for comparison, and statistical significance was represented as ***P < 0.001 vs control, ##P < 0.01 vs NE, P < 0.001, $$P < 0.01 vs Pre + NE.