Skip to main content
. 2023 Jun 20;22(13):1614–1636. doi: 10.1080/15384101.2023.2225924

Figure 4.

Figure 4.

PP1 activation impairs meiotic resumption. (a) Schematic representation of the experimental design. Prophase I oocytes were released from meiotic arrest into culture medium containing PDP-Nal (2.5–10 µM), 10 µM PDPm-Nal, or standard culture medium (Ctrl; 0 µM PDP-Nal). Nuclear envelope breakdown (NEBD) was monitored to track meiotic resumption. (b-c) Graphical representation of the percentage of oocytes to (b) resume meiosis (NEBD) or (c) die over time. n = 140–181 oocytes over 7–9 replicates. (d) Graphical representation of meiotic stage at oocytes death; n = 21–121 oocytes over 7–9 replicates. (e) Graphical comparison of pH3S10 fluorescence intensity. pH3S10 fluorescence was normalized to expression in Ctrl oocytes. Different letters denote a significant difference between the groups (Kruskal–Wallis test with Dunn’s post hoc). n = 13–41 oocytes over 2 replicates. Representative images of oocyte pH3S10 fluorescence, scale = 10 µm. (f) Graphical comparison of pH3T3 fluorescence intensity. pH3T3 fluorescence was normalized to expression in Ctrl oocytes. Different letters denote a significant difference between the groups (Kruskal–Wallis test with Dunn’s post hoc). n = 18–56 oocytes over 3–4 replicates. Representative images of oocyte pH3T3 fluorescence, scale = 10 µm. Scatter dot plots show each individual oocyte fluorescence intensity plus mean and SEM.