Figure 6.

PP1 activation from prometaphase to metaphase of meiosis I decreases oocyte survival and alters chromosome morphology. (a) Schematic representation of the experimental design. Prophase I oocytes were released from meiotic arrest and cultured for 3 h before treatment with increasing concentrations of PDP-Nal (2.5–40 µM), PDPm-Nal (10 µM) or standard culture medium (Ctrl; 0 µM PDP-Nal). (b) Graphical representation of the percentage of oocyte death over time. n = 29–106 oocytes over 2–5 replicates. (c) Graphical representation of percentage of oocytes dead with 5 h of PDP-Nal treatment at G/2 M (0–5 h; solid color) or during meiosis I (3–8 h; hashed color). * denotes a significant difference between 0–5 h and 3–8 h PDP-Nal treated oocytes (Fisher’s exact test) (d) Graphical representation of the percentage of oocytes with at least one pointy chromosome, with sample images of DAPI staining showing chromosome morphology. n = 34–59 oocytes over five replicates. * denotes a significant difference from PDPm-Nal control oocytes (Fisher’s exact test). Scatter dot plots show each individual oocyte fluorescence intensity plus mean and SEM.