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Western immunoblot of LPS preparations. Membranes were probed with MASF-Y5 or MASF-I as the primary antibody, and O antigens were detected by chemiluminescence. SFL124 (serotype Y) and S. flexneri Y53 (serotype 1a) were used as controls. LPS prepared from SFL1243 (SFL124 containing orf3) is recognized by both Y- and 1a-specific monoclonal antibodies, whereas LPS from SFL1244 (SFL124 containing orf1, orf2, and orf3) is recognized only by MASF-I.
