TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| Strains | ||
| E. coli | ||
| JM109 | supE44 gyrA96 recA1 relA1 endA1 thi hsdR17 Δ(lac-proAB) e14− (F′ traD36 proAB lacIqlacZΔM15) | 30 |
| B789 | JM109(pNV700) | This study |
| B799 | JM109(pNV710) | This study |
| B800 | JM109(pNV711) | This study |
| B801 | JM109(pNV712) | This study |
| S. flexneri | ||
| Y53 | Serotype 1a | SIDCa |
| Y340 | Serotype 1a | SIDC |
| Y829 | Serotype 1a | SIDC |
| SFL124 | ΔaroD, serotype Y | 16 |
| SFL1243 | SFL124(pNV711) | This study |
| SFL1244 | SFL124(pNV712) | This study |
| Plasmids | ||
| pUC18 | Cloning vector, Apr | 30 |
| pBluescript II KS | Cloning vector, Apr | Stratagene |
| pCR2.1 | Cloning vector, Kmr | Invitrogen |
| pNV394 | pPR691::Y53 genomic DNA | 1 |
| pNV462 | pUC19::5.8-kb HindIII fragment from pNV394 | 1 |
| pNV700 | pBluescript::5.8-kb EcoRI fragment from pNV394 | This study |
| pNV710 | pBluescript::4.8-kb SalI fragment from pNV394 | This study |
| pNV711 | pUC18::gtrI | This study |
| pNV712 | pUC18::orf1 bgt gtrI | This study |
a
SIDC, Swedish Institute for Infectious Disease Control, Stockholm, Sweden.