TABLE 2.
Sequence analysis of the 5.8-kb HindIII fragment of pNV394
| ORF or feature | Nt positiona | % GC | No. of amino acids encoded | Database search results
|
|
|---|---|---|---|---|---|
| Feature or protein (source; accession no.) | % Identity | ||||
| attP | 601–646 (3342–3387) | NAb | NA | attP core (SfV; U82619, U82620) | 100 |
| attP core (SfII; AF021347) | 100 | ||||
| attP core (P22; X04052) | 100 | ||||
| attP core (DLP12; M27155) | 100 | ||||
| orf1 | 848–1210 (3589–3951) | 42.7 | 120 | Orf2 (SfII; AF021347) | 98 |
| Orf6 (SfV; U82619, U82620) | 99 | ||||
| GtrA (SfX; AF056939) | 89 | ||||
| o120 (E. coli; ECAE000323) | 88 | ||||
| orf2 | 1207–2127 (3948–4868) | 42.0 | 306 | Bgt (SfII; AF021347) | 97 |
| Orf5 (SfV; U82619, U82620) | 97 | ||||
| GtrB (SfX; AF056939) | 95 | ||||
| o306 (E. coli; ECAE000323) | 89 | ||||
| orf3 | 2130–3650 (4871–6391) | 33.4 | 506 | No significant homology | |
| orf4c | 4387–3884 (7128–6625) | 39.7 | 167 | Orf3 (SfV; U82619, U82620) | 91 |
| Hypothetical 18.3-kDa protein (E. coli; ECAE000324) | 68 (133-amino-acid overlap) | ||||
| orf5′c | 4808–4296 (7549–7037) | 50.1 | 170 | Orf2 (SfV; U82619, U82620) | 94 (66-amino-acid overlap) |
a
The position relative to the 5.8-kb HindIII fragment is indicated. The nucleotide position relative to the complete 10.6-kb sequence is indicated in the parentheses.
b
NA, not applicable.
c
Present on complementary strand.