TABLE 3.
Sequence analysis of the regions up- and downstream of the 5.8-kb HindIII fragment
| Feature | Nt position (% GC) | Database search results
|
|
|---|---|---|---|
| Origin, feature, and gene (accession no.) | % Identity, overlap sizea | ||
| dsdCb | 353–54 (44.7) | E. coli dsdC (ECAE000324) | 93.4, 361 nt |
| 94.9, 99 aa (120 aa total) | |||
| IS629b | 1660–355 (53.9) | S. sonnei IS629 (X51586, X05953, X05954) | 98.9, 1,310 nt |
| E. coli IS3411 (M19532) | 98.6, 1,310 nt | ||
| IS600u | 1705–2968 (49.5) | S. flexneri plasmid pMYSH6000 (U82621) | 99.4, 1,264 nt |
| S. flexneri SBA1336 IS600-like element (U97492) | 99.4, 1,264 nt | ||
| S. sonnei IS600 (X05952) | 99.3, 1,264 nt | ||
| Shigella dysenteriae cytotoxin gene (M24352, M21947) | 99.3, 1,264 nt | ||
| IS600d | 7550–8813 (49.1) | S. flexneri SBA1336 IS600-like element (U97492) | 99.2, 1,264 nt |
| S. sonnei IS600 (X05952) | 99.1, 1,264 nt | ||
| S. dysenteriae cytotoxin gene (M24352, M21947) | 99.1, 1,264 nt | ||
| int′ | 8814–9782 (46.8) | SfV int (U82619, U82620) | 98.7, 972 nt |
| 97.5, 323 aa (387 aa total) | |||
| P22 int (X04052) | 76.9, 971 nt | ||
| 89.4, 321 aa (387 aa total) | |||
| SfII partial int (AF021347) | 98.7, 620 nt | ||
| 99.5, 205 aa | |||
| thrW tRNA geneb | 9872–9797 (50.9) | E. coli thrW (ECAE000132) | 100, 76 nt |
| attR | 9797–9842 | Refer to attP (Table 2) | 100, 45 nt attP (Table 2) |
| proAb | 10604–9987 (50.9) | E. coli proAB (ECAE000132) | 99, 616 nt |
| 98.5, 206-aa overlap with ProA (417 aa total) | |||
a
aa, amino acid.
b
Present on complementary strand.