Table 6.
Estimation of biological dose of the eight dose blinded samples (1–8) based on the yields of “dicentric + ring” and micronuclei. Four dose blinded samples were analyzed for scoring of “dicentric + ring” and remaining four were analyzed for micronuclei. 120 to 750 metaphases were analyzed for dicentrics (as per the yields obtained during the scoring) and more than 1000 binucleated cells were analyzed for micronuclei.
| S. No. | End Point | Blinded Sample | Dose Delivered (Gy) | Aberrations/Cells Scored | Aberration per Cell (Yield) | Estimated doses (Gy) | % variation in estimated doses |
|---|---|---|---|---|---|---|---|
| 1. | DCA | DCA-1 | 5.0 | 169/120 | 1.4083 | 5.79 {95% CI: 5.29–6.31} |
+16 |
| 2. | DCA | DCA-2 | 1.0 | 46/500 | 0.0920 | 1.01 {95% CI: 0.79–1.25} |
+0.9 |
| 3. | DCA | DCA-3 | 2.0 | 71/310 | 0.2290 | 1.91 {95% CI: 1.62–2.23} |
- 4.4 |
| 4. | DCA | DCA-4 | 0.5 | 4/750 | 0.0333 | 0.45 {95% CI: 0.31–0.62} |
- 9.94 |
| 5. | MN | MN-1 | 1.0 | 106/1071 | 0.0990 | 1.31 {95% CI: 1.16–1.47} |
+31.2 |
| 6. | MN | MN-2 | 0.25 | 17/1068 | 0.0153 | 0.31 {95% CI: 0.11–0.50} |
+22.8 |
| 7. | MN | MN-3 | 3.0 | 279/1037 | 0.2726 | 2.35 {95% CI: 2.20–2.51} |
- 21.7 |
| 8. | MN | MN-4 | 0.0 | 2/1120 | 0.0018 | 0 {95% CI: 0.0–0.0} |
0 |