TABLE 2.

Calculated net flux through phosphoglycerate mutase^a

Item	Data set 1 (DFY724 [wild type])		Data set 2 (DFY725 [gcr2])
	−2 min	10 min	−2 min	10 min
Vf	11.6 (11.1)	11.6 (9.72)	2.52 (3.02)	2.52 (4.75)
Vr	19.4 (20.2)	19.4 (25.6)	4.21 (3.51)	4.21 (1.85)
Glycerate-3-phosphate (mM)	0.94 (0.90)	0.97 (0.74)	0.92 (1.10)	4.31 (8.79)
Glycerate-2-phosphate (mM)	0.18 (0.19)	0.15 (0.20)	0.22 (0.18)	1.36 (0.66)
Ks (mM)	0.24 (0.25)	0.24 (0.31)	0.24 (0.20)	0.24 (0.11)
Kp (mM)	0.08 (0.076)	0.08 (0.06)	0.08 (0.095)	0.08 (0.16)
vf	6.34	7.36	1.27	1.25
vr	6.09	5.26	1.52	1.99
Net v (calculated)	0.25	2.10	−0.25	−0.73
Net v (measured)b	0.0	0.42	0.0	0.38

^aThe rate equations v_f = (V_fS/K_s)/D and v_r = (V_rP/K_p)/D, with D = 1 + S/K_s + P/K_p (where V_f and V_r are the V_max values for the forward and reverse reactions, respectively, and K_s and K_p are the K_ms for glycerate-3-phosphate and glycerate-2-phosphate, respectively), were applied, by using Microsoft EXCEL, to data from data sets 1 and 2 (Table 1), employing the K_ms from reference 12 and its V_r/V_f value of 1.66. The values in parentheses are recalculations using the ethanol flux (last line) as one of the six input values and allowing just one of the other six inputs to vary: i.e., for set 1, 10 min, a net v of 0.42 would have been obtained with a V_f of 9.72 instead of 11.6 μmol/min × mg of protein, or a V_r of 25.6 instead of 19.4 μmol/min × mg of protein, etc. V and v units are micromoles per minute per milligram of protein. 

^bEthanol flux = v_glucose × (ethanol/glucose).